Are there significant differences in the gut microbiome of East Kent patients before and after they undergo cholecystectomy in the last year? A Prospective Cohort Study.
DOI:
https://doi.org/10.22024/UniKent/03/ajpp.1372Resumen
Background: The role of the gut microbiome in gallstone disease remains a growing area of research. Previous research into this area includes small scale studies but the current knowledge of specific microbial species and functional pathways leading to gallstone disease is still limited. Since gallstone disease remains a significant problem in the NHS, leading to debilitating symptoms and partially caused by environmental and social factors, which continue to become more challenging in the UK, this research aimed to address this area of concern. This type of research could potentially lead to microbial dysbiosis becoming a recognised risk factor for gallstone disease and being considered when formulating management plans for patients.
Methods: The methods used for this study involved obtaining stool samples from patients scheduled for cholecystectomy, samples from the same patients after cholecystectomy as well as six months after cholecystectomy. The DNA extraction protocol included with PureLink Microbiome DNA Purification Kit was followed to extract and purify microbial DNA from these samples. This purified DNA underwent PCR to amplify the amount of 16S microbial DNA before undergoing gel electrophoresis to verify the presence of bacterial DNA. The DNA was then sent for sequencing and statistical analysis using Muther and QIIME2 software was used to investigate patterns in the results.
Results: The results showed values of DNA concentrations from the extraction products as well as a comparison of DNA concentration values at three different time points: before, after and 6 months after cholecystectomy. They revealed a high degree of variability in these values. The results also show the change in DNA yield across the three time points and a comparison of the DNA yield before and after using Phosphate-Buffered Saline (PBS) to optimise the DNA extraction protocol, as well as a figure showing a gel.
Conclusions: This figure demonstrated the presence of 16S DNA, demonstrating that the DNA extraction was successful.
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
© The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
AJPP requests that, as the creator(s)/author(s) of the manuscript you are submitting, that you assign certain rights to the manuscript to the AJPP in exchange for undertaking to publish the article in electronic form and, in general, to pursue its dissemination throughout the world. The rights the AJPP requests are:
- The right to publish the article in electronic form or in any other form it may choose that is in keeping with its role as a scholarly journal with the goal of disseminating the work as widely as possible;
- The right to be the sole publisher of the article for a period of 12 months;
- The right to make the article available to the public within a period of not more than 24 months, as determined by relevant journal staff of the AJPP;
- The right to grant republication rights to itself or others in print, electronic, or any other form, with any revenues accrued to be shared equally between the author(s) and the journal;
- The right to administer permission to use portions of the article as requested by others, seeking recompense when the AJPP sees it as warranted;
- The right to seek or take advantage of opportunities to have the article included in a database aimed at increasing awareness of it;
- As the author(s), the AJPP wishes you to retain the right to republish the article, with acknowledgement of the AJPP as the original publisher, in whole or in part, in any other pbulication of your own, including any anthology that you might edit with up to three others;
- As the author(s), the AJPP wishes you to retain the right to place the article on your personal Web page or respository of your university or institution. The AJPP askes that you include this notice: A fully edited, peer-reviewed version of this article was first published by the Advanced Journal of Professional Practice, <Year>, <Volume>, <Issue>, <Page Numbers>.
- You retain the right to unrestricted use of your paper for yourself or for your own teaching purposes.
BY AGREEING TO THE FOREGOING, YOU CONFIRM THAT THE MANUSCRIPT YOU ARE SUBMITTING HAS NOT BEEN PUBLISHED ELSEWHERE IN WHOLE OR IN PART, AND THAT NO AGREEMENT TO PUBLISH IS OUTSTANDING.
SHOULD THE ARTICLE CONTAIN MATERIAL WHICH REQUIRES WRITTEN PERMISSION FOR INCLUSION, YOU AGREE THAT IT IS YOUR OBLIGATION IN LAW TO IDENTIFY SUCH MATERIAL TO THE EDITOR OF THE AJPP AND TO OBTAIN SUCH PERMISSION. THE AJPP WILL NOT PAY ANY PERMISSION FEES. SHOULD THE AJPP BE OF THE OPINION THAT SUCH PERMISSION IS NECESSARY, IT WILL REQUIRE YOU TO PURSUE SUCH PERMISSSION PRIOR TO PUBLICATION.
AS AUTHOR(S), YOU WARRANT THAT THE ARTICLE BEING SUBMITTED IS ORIGINAL TO YOU.
Provided the foregoing terms are satisfactory, and that you are in agreement with them, please indicate your acceptance by checking the appropriate box and proceed with your submission.
